Gene expression in human NAFLD.

MFn1, MFn2 and OPA1 gene expression of cardiac tissue after high intensity interval training and resveratrol injection in NAFLD rats

Background: Non‐alcoholic fatty liver disease (NAFLD) is associated with an increased risk of cardiovascular disease. The aim of the present study was to examine the effect high intensity interval training (HIIT) with resveratrol supplementation on Cardiomyocytes expression of MFn1, MFn2 and OPA1 in NAFLD rats. Materials and methods: In this experimental study, forty-eight Wistar male rats wer...

Effect of Eight Weeks of Compound Exercises on Human Body in Obese Males with Non-Alcoholic Fatty Liver Disease with Emphasis on Methylenetetrahydrofolate Reductase Gene Polymorphism

Background and purpose: Non-alcoholic fatty liver disease (NAFLD) is the most common liver disorder in the world. Genetic factors are believed to be associated with development of NAFLD. This research aimed at studying the effect of eight weeks of compound exercises (aerobic and resistance) on human body with three genotypes (TT, CT, CC) of C677T polymorphism of methylenetetrahydrofolate reduct...

A COMPARATIVE STUDY BETWEEN EXPRESSION OF A SYNTHETIC GENE OF HUMAN BASIC FIBROBLAST GROWTH FACTOR (hbFGF) AND ITS RELATED cDNA IN ESCHERICHIA COLI

The gene encoding the human basic fibroblast growth factor (hbFGF) has been already chemically-synthesized and cloned in pET-3a expression vector (Pasteur Institute of Iran). In the present study, we compared the level of expression of this synthetic hbFGF and its related cDNA in Escherichia coli. The pBR322-cDNA of hbFGF supplied by Dr. Seno (from Molecular Biology Dept, Okaido prefectural uni...

Inhibition of 5-Lipoxygenase inhibitor zileuton in high-fat diet-induced nonalcoholic fatty liver disease progression model

Objective(s): Arachidonic Acid/5-lipoxygenase (AA/5-LOX) pathway connects lipid metabolism and proinflammatory cytokine, which are both related to the development and progression of nonalcoholic fatty liver disease (NAFLD). Therefore, the present study was designed to investigate the role of AA/5-LOX pathway in progression of NAFLD, and the effect of zileuton, an inhibitor of 5-LOX, in this mod...

Effect of Medium-Chain Triglycerides on Gene Expression of ATP-Citrate Lyase and H3K27ac in Developing Non-Alcoholic Fatty Liver Disease in an Animal Model

Background and purpose: Increase in ATP-dependent Citrate Lyase (ACLY) is observed in individuals with nonalcoholic fatty liver disease (NAFLD). One of the compounds considered for the prevention of fatty liver is Medium chain triglycerides (MCT). In this study, the preventive effect of MCT on ACLY enzyme gene expression and the level of histone 3 lysine 27 acetylation (H3K27ac) were investigat...

The Role of Bacterial Vectors in the Expression of Human IFN-y Gene